circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)  (Arraystar inc)

Journal: Frontiers in Neuroscience

Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

doi: 10.3389/fnins.2020.00081

Figure Lengend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

Techniques: Expressing, Control, Quantitative Proteomics

Journal: Frontiers in Neuroscience

Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

doi: 10.3389/fnins.2020.00081

Figure Lengend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

Techniques: Quantitative RT-PCR, Microarray, Quantitative Proteomics

Journal: Frontiers in Neuroscience

Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

doi: 10.3389/fnins.2020.00081

Figure Lengend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

doi: 10.3389/fnins.2020.00081

Figure Lengend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

doi: 10.3389/fnins.2020.00081

Figure Lengend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

Techniques:

Journal: RSC Advances

Article Title: The potential role of circRNA_004229 in hair/epidermal regulation after MED1 ablation in keratinocytes †

doi: 10.1039/c9ra02322d

Figure Lengend Snippet: The simple explanation of circRNA_004229 and its regulation mechanisms from circRNA to miRNA to mRNA.

Article Snippet: Subsequently, the cRNAs were hybridized using Arraystar Mouse circRNA Array (8 × 15K, Arraystar Inc).

Techniques:

Journal: RSC Advances

Article Title: The potential role of circRNA_004229 in hair/epidermal regulation after MED1 ablation in keratinocytes †

doi: 10.1039/c9ra02322d

Figure Lengend Snippet: Heat maps of the differentially expressed circRNAs in dorsal epidermis from MED1 KO mice compared with WT mice. (A) The cluster heat map showed all differentially expressed circRNAs in MED1 KO mice vs. WT mice (FC > 2.0 and p -value < 0.05). (B) The hierarchical clustering of the top 15 up- and down-regulated circRNAs in MED1 KO mice vs. WT mice. Rows represent circRNAs, while columns represent compared groups. The expression index is colored in red indicating high expression level, and in green indicating low expression level. The red arrow indicates circRNA_004229.

Article Snippet: Subsequently, the cRNAs were hybridized using Arraystar Mouse circRNA Array (8 × 15K, Arraystar Inc).

Techniques: Expressing

Journal: RSC Advances

Article Title: The potential role of circRNA_004229 in hair/epidermal regulation after MED1 ablation in keratinocytes †

doi: 10.1039/c9ra02322d

Figure Lengend Snippet: Mmu_circRNA_004229 was down-regulated and promoted transcriptional inhibition of Perp and Tnfrsf19. (A) QRT-PCR validation for mmu_circRNA_004229 (a) and mRNA levels of Perp (b) and Tnfrsf19 (c) and GAPDH expression was used as a control. (B) TUNEL assay of WT mice (a) and MED1 KO mice (b), the red arrows indicate the apoptosis cells. (c) Quantitative results of TUNEL assay ( n = 3, * P < 0.05).

Article Snippet: Subsequently, the cRNAs were hybridized using Arraystar Mouse circRNA Array (8 × 15K, Arraystar Inc).

Techniques: Inhibition, Quantitative RT-PCR, Expressing, TUNEL Assay

Journal: RSC Advances

Article Title: The potential role of circRNA_004229 in hair/epidermal regulation after MED1 ablation in keratinocytes †

doi: 10.1039/c9ra02322d

Figure Lengend Snippet: The biding site prediction and network construction of mmu_circRNA_004229. (A) Binding sites of miR-149-5p/miR-207 in 3′-UTR of mmu_circRNA_004229. (B) Specific network of mmu_circRNA_004229-miR-207/149-5p-targets. We chose targets according to target score ≥80 in miRDB database. We used Cytoscape software 3.5.0 to visualize circRNA_004229-miRNA-targets interactions. In the network, the red rectangle represents circRNA_004229; the yellow rectangle represents miRNA-207/149-5p; the blue oval represents target genes of miRNA-207/149-5p and the pink oval represents Perp/Tnfrsf19. The relationship between the nodes was connected with solid lines.

Article Snippet: Subsequently, the cRNAs were hybridized using Arraystar Mouse circRNA Array (8 × 15K, Arraystar Inc).

Techniques: Binding Assay, Software